![]() ![]() The following tests and methods are consistent for the accuracy, detection limit, and precision testing and, therefore, are described first. The objective of this study was to estimate the performance characteristics (accuracy, detection limit, and precision) of commercially available ELISA and AGID kits from the USA in relation to the officially approved reference AGID assay in Canada, and to determine if, in the current Canadian context, the ELISA could also be adopted as an official test method for the diagnosis and control of EIA in this country. Validation studies have indicated excellent agreement between these ELISAs and the AGID assay ( 4, 5), with the ELISA being found, in some cases, to be even more sensitive than the AGID ( 6– 9). In the United States (USA), a few ELISAs have been approved by the US Department of Agriculture Animal and Plant Health Inspection Service (USDA:APHIS) in the 1990’s as equivalent test methods for the diagnosis of EIA ( 4, 5). This test, specific to the p26 core viral protein, is relatively rapid, inexpensive, simple, and highly specific to identify animals infected with the EIA virus, although it is interpreted subjectively by visual reading of precipitation line curvature.ĭuring the last few years, the detection of EIA antibodies by ELISA has been described and used in some countries where this test is commercialized under various formats. The agar gel immunodiffusion test (AGID) ( 2), prescribed by the Office International des Épizooties (OIE) for international trade ( 3), is currently in use in Canada for its EIA control program. Seropositivity is a good indication of infection because horses infected with the EIA virus carry it for life ( 1), with the development of a sustained antibody response appearing usually within 45 d of viral infection ( 2). The diagnosis of EIA in horses is done serologically. Following a positive status, an infected animal is usually euthanized and contact horses are put under a federally-imposed quarantine until confirmation of their negative status. In this country, horse EIA monitoring is encouraged for attendance at races, shows, fairs, sales, breeding farms, etc. ![]() In Canada, this reportable disease falls under the Health of Animals Regulations sponsored by the Canadian Food Inspection Agency (CFIA). La qualité des caractéristiques de performances des ELISAs dirigés contre les anticorps spécifiques à la p26 favorisent l’implantation de ceux-ci à des fins réglementaires au Canada.Įquine infectious anemia (EIA) is a retroviral disease of all equidae, including horses, mules, and donkeys, that falls under a regulatory control program in many industrialized countries. La trousse ELISA dirigée contre les anticorps spécifiques à la protéine trans-membranaire gp45 révélait une sensibilité relative réduite. Les 3 trousses ELISA dirigées contre les anticorps spécifiques à la protéine nucléaire p26 offraient une très bonne performance en comparaison avec l’IDG de référence, avec une excellente exactitude et une précision acceptable. Les trousses commerciales d’IDG se sont avérées équivalentes pour le diagnostic sérologique de l’AIÉ. Au total 285 échantillons positifs et 315 échantillons négatifs au test IDG de référence ont été testés à l’aveugle avec 2 autrestrousses IDG et 4 trousses ELISA. The performance characteristics of the ELISAs directed against antibodies to p26 are, therefore, adequate to support the implementation of ELISA for regulatory purposes in Canada.Ĭette étude avait pour but d’estimer les caractéristiques de performance (exactitude, limite de détection et précision) de trousses d’épreuve immunoenzymatique (ELISA) et d’immunodiffusion en gel d’agar (IDG) disponibles commercialement en les comparant avec une trousse IDG utilisée présentement à des fins réglementaires au Canada pour la détection d’anticorps dirigés contre l’anémie infectieuse deséquidés (AIÉ). The single ELISA directed against antibodies to the gp45 trans-membrane viral protein yielded a lower relative sensitivity. The 3 ELISAs directed against antibodies to the p26 core protein also performed relatively well in comparison with the reference AGID, with excellent relative accuracy and acceptable precision. Commercially available AGID kits for the serodiagnosis of EIA were found equivalent. A total of 285 positive and 315 negative samples by the reference AGID were tested blindly on 2 other AGID and 4 ELISA kits. The purpose of this study was to estimate the performance characteristics (accuracy, detection limit, and precision) of commercially available enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) kits in comparison with a reference AGID kit for the detection of equine infectious anemia (EIA) antibodies in horses for regulatory use in Canada. ![]()
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |